VetSRev
Database of veterinary systematic reviews
Database of veterinary systematic reviews
Tropical Medicine & International Health : TM & IH (2015) 20:
DOI: 10.1111/tmi.12429
OBJECTIVE: To evaluate the quality of serological diagnosis of canine visceral leishmaniasis in the Americas. METHODS: A systematic review found original studies in the databases MEDLINE, EMBASE and LILACS up to November 2012 and in complementary sources up to February 2013. Studies were evaluated in accordance with QUADAS 2 and STARD parameters and recommended in accordance with GRADE parameters. Meta-analysis was carried out with MetaDiSc software, using the random effect model. RESULTS: 284 studies were identified, of which 25 met the inclusion criteria, comprising the final synthesis. All but one were done in Brazil, and only 2 were judged to be of good quality. 15 studies involving immuno-enzymatic tests with crude antigens (cELISA), 11 studies on indirect immunofluorescence tests (IFAT) and 3 on the immunochromatographic dual path platform (DPP) test were meta-analysed. The combined results for sensitivity and specificity were: cELISA: 0.89 (CI 95% 0.87 to 0.91) and 0.87 (CI 95% 0.86 to 0.88); IFAT: 0.88 (CI 95% 0.85 to 0.91) and 0.63 (CI 95% 0.61 to 0.65) and DPP: 0.83 (CI 95% 0.78 to 0.88) and 0.73 (CI 95% 0.70 to 0.75). CONCLUSION: cELISA and DPP tests have moderate accuracy for the diagnosis of canine visceral leishmaniasis, and the quality of the design, implementation and analysis of validation studies on diagnostic tests for this disease urgently require improvement. The recommendation for use of the evaluated tests is based on evidence that is scarce and restricted to Brazil.
Peixoto, H. M., de Oliveira, M. R., & Romero, G. A. (2015). Serological diagnosis of canine visceral leishmaniasis in brazil: systematic review and meta-analysis. Tropical Medicine & International Health : TM & IH, 20(3). https://doi.org/10.1111/tmi.12429 Dogs, Leishmaniasis