Role of exogenous antioxidants on the performance and function of pig sperm after preservation in liquid and frozen states: A systematic review

Ribas-Maynou, J. and Mateo-Otero, Y. and Delgado-Bermúdez, A. and Bucci, D. and Tamanini, C. and Yeste, M. and Barranco, I.

Theriogenology (2021) 173: 279–294

DOI: 10.1016/j.theriogenology.2021.07.023

Abstract

In situations where an excessive generation of reactive oxygen species overwhelms antioxidant capacity, a harmful effect on sperm function is exerted. Antioxidants are molecules capable of minimizing this detrimental effect, which is important in pig sperm due to the high content of polyunsaturated fatty acids in their plasma membrane. The present systematic review aims at evaluating whether supplementing semen extenders (for liquid storage at 17 °C) or freezing and/or thawing media (for cryopreservation) with antioxidants influences sperm quality and functionality parameters, and in vitro/in vivo fertility outcomes. We defined inclusion and exclusion criteria in a PICOS table according to PRISMA guidelines, and conducted a literature search through MEDLINE-PubMed in November 2020. After systematic selection, 75 studies were included: 47 focused on cryopreservation and 28 on liquid storage at 17 °C. More than 70% of the studies included in this review showed that adding semen extenders for liquid storage and/or freezing/thawing media for cryopreservation with antioxidants enhances sperm quality and functionality parameters. In addition, this supplementation improves in vivo/in vitro fertility outcomes, supporting the hypothesis that the beneficial effect observed upon sperm quality has a positive impact on reproduction outcomes.

Citation

Ribas-Maynou, J., Mateo-Otero, Y., Delgado-Bermúdez, A., Bucci, D., Tamanini, C., Yeste, M., & Barranco, I. (2021). Role of exogenous antioxidants on the performance and function of pig sperm after preservation in liquid and frozen states: A systematic review. Theriogenology, 173, 279–294. https://doi.org/10.1016/j.theriogenology.2021.07.023 *Antioxidants, *Semen Preservation/veterinary, Animals, Antioxidants, Cryopreservation, Cryopreservation/veterinary, Cryoprotective Agents/pharmacology, Freezing, Liquid-storage, Male, Pig, Semen Analysis/veterinary, Sperm, Sperm Motility, Spermatozoa, Swine

Keywords

• *Antioxidants
• *Semen Preservation/veterinary
• Animals
• Antioxidants
• Cryopreservation
• Cryopreservation/veterinary
• Cryoprotective Agents/pharmacology
• Freezing
• Liquid-storage
• Male
• Pig
• Semen Analysis/veterinary
• Sperm
• Sperm Motility
• Spermatozoa
• Swine