[Principal molecular markers used to identify Babesia bovis and Babesia bigemina]

Rios T, S and Rios O, L

Revista MVZ Cordoba (2011) 16: 2470–2483

Link: http://www.scielo.org.co/scielo.php?pid=S0122-02682011000200004&script=sci_arttext

Abstract

This paper describes the principal molecular markers used to identify _B. bovis_ and _B. bigemina_ reported in the scientific literature. A systematic review was designed based on the application of the PICO methodologic modified strategy to define the nucleotide sequences detected in different geographical locations and their diagnostic utility. An advanced search was made in data bases ScienceDirect, SpringerLink and PubMed. Using the terms "Babesia bovis" and "DNA" and "Babesia bigemina" and "DNA". A total of 68 original articles were selected after the information was filtered. Both included and excluded articles were registered in tables, where its position of their status, within the study, was represented. The 68 articles were evaluated using a previously defined criteria of inclusion or exclusion. A total of 21 articles met the inclusion criteria and were included in this study. It was described the usefulness of molecular markers referenced in the scientific literature from 1995 to 2010: small-subunit ribosomal rna, cytochrome b gene, msa-1 and msa-2c gene, bv gene, elongation factor-1 alpha (ef-1 alpha ), beta-tubulin gene, sbp 1-2-3, and rap genes, its diagnostic application and its use in different geographical locations. Molecular markers used for detection of bovine babesia vary by geographic region, degree of genetic conservation and results of previous studies that conclude their diagnostic utility.

Citation

Rios T, S., & Rios O, L. (2011). [Principal molecular markers used to identify Babesia bovis and Babesia bigemina]. Revista MVZ Cordoba, 16(2), 2470–2483. http://www.scielo.org.co/scielo.php?pid=S0122-02682011000200004&script=sci_arttext genetic markers, [Indexed using CAB Thesaurus terms], animals, Artiodactyla, Chordata, eukaryotes, mammals, vertebrates, Bos, Bovidae, cattle, Diagnosis of Animal Diseases [LL886], ruminants, DNA, Helminth, Mollusc and Arthropod Parasites of Animals [LL822], Protozoan, invertebrates, protozoal infections, diagnosis, diagnostic techniques, genes, databases, data banks, geographical distribution, Protozoa, Apicomplexa, Babesia, Babesia bigemina, Babesia bovis, Babesiidae, babesiosis, beta-tubulin, cytochrome b, deoxyribonucleic acid, diagnostic value, DNA sequences, Genetics and Molecular Biology of Microorganisms [ZZ395], identification, nucleotide sequences, Piroplasmorida, protozoal diseases, red water, ribosomal RNA, rRNA, tick fever, tickborne diseases

Keywords

• genetic markers
• [Indexed using CAB Thesaurus terms]
• animals
• Artiodactyla
• Chordata
• eukaryotes
• mammals
• vertebrates
• Bos
• Bovidae
• cattle
• Diagnosis of Animal Diseases [LL886]
• ruminants
• DNA
• Helminth
• Mollusc and Arthropod Parasites of Animals [LL822]
• Protozoan
• invertebrates
• protozoal infections
• diagnosis
• diagnostic techniques
• genes
• databases
• data banks
• geographical distribution
• Protozoa
• Apicomplexa
• Babesia
• Babesia bigemina
• Babesia bovis
• Babesiidae
• babesiosis
• beta-tubulin
• cytochrome b
• deoxyribonucleic acid
• diagnostic value
• DNA sequences
• Genetics and Molecular Biology of Microorganisms [ZZ395]
• identification
• nucleotide sequences
• Piroplasmorida
• protozoal diseases
• red water
• ribosomal RNA
• rRNA
• tick fever
• tickborne diseases