VetSRev
Database of veterinary systematic reviews
Database of veterinary systematic reviews
Research in Veterinary Science (2021) 135: 72–77
DOI: 10.1016/j.rvsc.2021.01.004
Paratuberculosis is an incurable infectious disease that affects several species, including goat (Capra hircus). The etiologic agent is Mycobacterium avium subspecies paratuberculosis (MAP) that has tropism for the intestine, causing anorexia, progressive weight loss and death. In goats, the main transmission route is the ingestion of water and food contaminated by infected feces. Affected animals also eliminate the agent through milk, with a potential biological risk to public health. Thus, the aim of this study was to conduct a research of the literature available in electronic media for a systematic review, followed by a meta-analysis of the results found on prevalence and diagnostic tests adopted in the detection of MAP antibodies and DNA in goat milk. The following search parameters were used: "Mycobacterium avium subsp. paratuberculosis" AND (goat OR small ruminant) AND (milk OR pasteurized milk). Strictly obeying pre-established criteria, 437 articles were selected from the respective electronic databases of scientific content: ScienceDirect (285), PubMed (68), Web of Science (60) and Scopus (24), of which nine papers were elected to the construction of the systematic review and meta-analysis. The prevalence of MAP antibodies in milk detected by milk-ELISA ranged from 1.1 to 67.7% and the prevalence of MAP DNA in goat milk detected by MAP-specific polymerase chain reaction (PCR) ranged from 1.94 to 37.74%. A meta-analysis indicated a combined MAP infection prevalence of 8.24%, but with high heterogeneity among study findings (I^\textrm2 = 98.7%). The identification of the MAP in goat milk implies the need for surveillance of the agent in order to prevent economic losses and impact on public health.
Roberto, J. P. de L., Limeira, C. H., Barnabe, N. N. da C., Soares, R. R., Silva, M. L. C. R., Gomes, A. A. de B., Higino, S. S. dos S., Azevedo, S. S. de, & Alves, C. J. (2021). Antibody detection and molecular analysis for Mycobacterium avium subspecies paratuberculosis (MAP) in goat milk: systematic review and meta-analysis. Research in Veterinary Science, 135, 72–77. https://doi.org/10.1016/j.rvsc.2021.01.004 Animals, Female, mammals, Johne’s disease, Paratuberculosis, Goats, Animal Immunology [LL650], Diagnosis of Animal Diseases [LL886], Host Resistance and Immunity [HH600], meta-analysis, DNA, techniques, systematic reviews, Dairy Animals [LL110], data analysis, health, intestines, disease prevalence, milk, Milk and Dairy Produce [QQ010], bacterium, public health, Milk, wild animals, Mycobacterium, infections, diagnosis, diagnostic techniques, bacterial diseases, detection, goat milk, milk yield, databases, milk products, goats, milk yielding animals, data banks, heterogeneity, contamination, Public health, communicable diseases, infectious diseases, pasteurization, pasteurizing, surveillance, incidence, Mycobacterium avium, bacterial infections, bacterioses, deoxyribonucleic acid, Genetics and Molecular Biology of Microorganisms [ZZ395], antibodies, dairy products, Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821], Molecular Biology and Molecular Genetics [ZZ360], Food Contamination, Residues and Toxicology [QQ200], tests, faeces, feces, losses, Milk/*microbiology, pasteurized milk, Pcr, milk-yielding animals, anorexia, Antibodies, Bacterial/*isolation & purification, antibody detection, antibody testing, antibody tests, Capra, Goat Diseases/diagnosis/*microbiology, Goats/genetics, inappetence, Milk-ELISA, Mycobacterium avium subsp paratuberculosis, Mycobacterium avium subsp. paratuberculosis/*immunology, paratuberculosis, Paratuberculosis/microbiology, weight losses, wild goats