Candidate genes for tick resistance in cattle: a systematic review combining post-GWAS analyses with sequencing data

Santos, C. G. dos and Sousa, M. F. and Vieira, J. I. G. and Morais, L. R. de and Fernandes, A. A. S. and Littiere, T. de O. and Otto, P. I. and Machado, M. A. and Silva, M. V. G. B. and Bonafe, C. M. and Magalhaes, A. F. B. and Verardo, L. L.

Journal of Applied Animal Research (2022) 50: 460–470

DOI: 10.1080/09712119.2022.2096035

Abstract

Rhipicephalus microplus causes huge losses in cattle. Host genetic background greatly affects the immune efficiency in resistance or susceptibility to tick infestation, which is one of the many factors that play a role on that trait. We performed a systematic review of genome-wide association studies (GWAS) for tick resistance in cattle resulting in 1353 candidate genes for post-GWAS analyses. From those, genes showing possible structural variants from the bovine genome were classified by the Variant Effect Predictor from Ensembl. Ninety-two candidate genes showed potential structural variants in 5’ UTR and coding region and were used for functional annotation. Enriched biological processes (e.g. regulation of eosinophil chemotaxis, RIG-I signalling pathway and monocyte differentiation) and candidate genes (e.g. DAPK2, PUM1, ACIN1, INPP5D) linked with immune system function were identified and thus associated with tick resistance. Besides, gene-transcription factors (TFs) networks were obtained from TFs associated with immune system (FOXO3, PPARG, STAT3, NFKB1, GATA3 and ARNT) and the candidate genes associated with tick resistance in cattle highlighted (e.g. OR4L1, PNP, LRRIQ1, GIMAP8, MYO6, MEP1A and LRFN2). Thus, promising candidate genes with a possible functional role for tick resistance in cattle are presented for further in vitro and/or in vivo analyses.

Citation

Santos, C. G. dos, Sousa, M. F., Vieira, J. I. G., Morais, L. R. de, Fernandes, A. A. S., Littiere, T. de O., Otto, P. I., Machado, M. A., Silva, M. V. G. B., Bonafe, C. M., Magalhaes, A. F. B., & Verardo, L. L. (2022). Candidate genes for tick resistance in cattle: a systematic review combining post-GWAS analyses with sequencing data. Journal of Applied Animal Research, 50(1), 460–470. https://doi.org/10.1080/09712119.2022.2096035 immune system, Cattle, systematic reviews, in vitro, Ticks, genes, gene expression, genomes, transcription, infestation, nucleotide sequences, resistance, transcription factors, susceptibility

Keywords

• immune system
• Cattle
• systematic reviews
• in vitro
• Ticks
• genes
• gene expression
• genomes
• transcription
• infestation
• nucleotide sequences
• resistance
• transcription factors
• susceptibility