Factors affecting laboratory production of buffalo embryos: a meta-analysis

Suresh, K P and Nandi, S and Mondal, S

Theriogenology (2009) 72: 978–985

DOI: 10.1016/j.theriogenology.2009.06.017

Abstract

In vitro fertilization (IVF) provides an excellent and inexpensive source of embryos for carrying out basic research on developmental physiology, farm animal breeding, and for commercial applications. Meta-analysis of the results from different publications rather than a narrative review may provide a current status of this technology in buffalo (Bubalus bubalis). In order to gain an idea of the factors affecting the IVF in buffalo, a review of the various studies conducted on buffalo IVF and a meta-analysis of their findings was undertaken. More than 100 articles published from 1991 to 2008 were searched, and results were subjected to meta-analysis to determine the treatment variations without any bias. Thirty factors affecting in vitro embryo production in buffalo were considered. Initially, both fixed- and random-effect models were used. We did not observe any heterogeneity between the studies. Thereafter, all the studies were pooled using the fixed-effect model for analysis. Our analysis suggested that good buffalo oocytes with more than three to five cumulus layers recovered from large-sized follicles in cold seasons when cultured in TCM-199 supplemented with serum, follicle-stimulating hormone, and cysteamine resulted in maximum maturation rate and subsequent embryonic development after insemination. The values obtained in the current study may be considered for a simulation model in establishing a cost-effective suitable method for buffalo IVF in further planned research.

Citation

Suresh, K. P., Nandi, S., & Mondal, S. (2009). Factors affecting laboratory production of buffalo embryos: a meta-analysis. Theriogenology, 72(7), 978–985. https://doi.org/10.1016/j.theriogenology.2009.06.017 Animals, Male, Female, Embryonic Development/physiology, Culture Media, Buffaloes/embryology, Cells, Cultured, Cysteamine/administration & dosage, Embryo Culture Techniques, Fertilization in Vitro/methods/veterinary, Follicle Stimulating Hormone/administration & dosage, Oocytes/physiology, Ovarian Follicle/cytology

Keywords

• Animals
• Male
• Female
• Embryonic Development/physiology
• Culture Media
• Buffaloes/embryology
• Cells
• Cultured
• Cysteamine/administration & dosage
• Embryo Culture Techniques
• Fertilization in Vitro/methods/veterinary
• Follicle Stimulating Hormone/administration & dosage
• Oocytes/physiology
• Ovarian Follicle/cytology